An ultra-performance LC–MS/MS method for determination of JRF103 in human plasma: application in first in-patient study

Jinhttps Y, Dihttps X, Fuhttps L et al. | Bioanalysis, 14(17), 1165-1175, (2022) Keywords: • cancer • JRF103 • method validation • pharmacokinetics • UPLC–MS/MS Background: JRF103, a novel pan-HER inhibitor, has shown potent activity against HER1, HER2, HER4 and EGFR in vitro. To support its first in-patient trial, a sensitive and rapid method was developed and validated using ultra-performance LC–MS/MS. Materials & methods: JRF103 was extracted from plasma using protein precipitation. Extracts were subjected to ultra-performance LC–MS/MS with electrospray ionization. Results: Separation of analyte was achieved using a 1.7-μm C18 column (2.1 × 50-mm internal diameter) with a gradient...