Accelerating isotope dilution LC-MS-based desmosine quantification for estimating elastin turnover

Kuzmanova E, McIntyre A, Syed M et al. | Bioanalysis, 17(02), 99–104, (2025) Keywords: • desmosine • microwave • micro solid phase extraction • stable isotope dilution LC-MS/MS • aortic dissection • aortic aneurysm Aims: Circulating total desmosine, representing endogenous systemic elastin degradation activity, is an emerging biomarker for mortality risk in several diseases and aging. However, the existing analytical method takes more than 23 hours to complete, limiting its potential applications. The objective of this study was to shorten the turnover time of a stable isotope dilution liquid chromatogram mass spectrometry-based desmosine assay. Materials & methods: Plasma samples were analyzed...