A workflow for the metabolomic/metabonomic investigation of exhaled breath using thermal desorption GC–MS

Background: Confounding factors in the analysis of human breath by thermal desorption GC–MS are reviewed, with special emphasis on the high water levels encountered in human breath samples. Results: Multilinear regression optimization of breath sampling factors, along with the selection of ubiquitous sample components used as retention-time standards, enabled data registration based on retention indexing and mass spectral alignment. This was done on a component-by-component basis. The methodology developed reconciled participant safety, artefacts from accelerated hydrolysis of the stationary phase and the destructive nature of thermal desorption. Furthermore, using ubiquitous methylated cyclic-siloxanes in the thermal desorption-GC–MS chromatograms enabled secondary retention...