A whole blood microsampling furosemide assay: development, validation and use in a pediatric pharmacokinetic study

Bamat N, Vedar C, Reilly M, Moorthy G & Zuppa A | Bioanalysis, 14(15), 1025-1038 (2022) Keywords: •  furosemide • human whole blood • LC-MS/MS • pediatrics • pharmacokinetics • volumetric absorptive microsampling Background: Furosemide is a commonly used diuretic for the treatment of edema. The pharmacokinetics of furosemide in neonates as they mature remains poorly understood. Microsampling assays facilitate research in pediatric populations. Results: We developed and validated a liquid chromatography–tandem mass spectrometry method for the quantitation of furosemide in human whole blood with volumetric absorptive microsampling (VAMS) devices (10 μl). Furosemide was stable in human whole blood VAMS under the study's assay conditions. This work established stability for...