A hybrid IA–LC–MS/MS method for adrenocorticotropic hormone(1–24) to support interpretation of low-dose cosyntropin-stimulation test

Jones BR, Shao J, Sanghvi M, Ayala A, Luo R & Wang J | Bioanalysis, 16(14), 711–720, (2024) Keywords: • ACTH(1–24) • ACTH(1–39) • bead conjugation • cosyntropin-stimulation test (CST) • fit-for-purpose • hybrid IA–LC–MS Adrenocorticotropic hormone 1–24 (ACTH[1–24]) has a similar effect as endogenous ACTH(1–39) to generate cortisol by targeting the MC2R receptor on the adrenal gland. A new investigational ACTH receptor antagonist drug is being developed to treat diseases of ACTH excess (e.g., Cushing's disease) by binding to the MC2R receptor. Administration of ACTH(1–24) was used in a Phase I clinical study to assess the ability of this...