Flow cytometry in cell-based pharmacokinetics or cellular kinetics in adoptive cell therapy


Pharmacokinetics (PK) defines the disposition of a drug in the body based primarily on measurements in fluids or tissues by various bioanalytical methods during the drug development. Most bioanalytical methods utilize ligand-binding or LC–MS technology, and the assays are validated and applied following the guidelines from various regulatory agencies in their countries (e.g., US FDA, EMEA or Health Canada) [1]. These bioanalytical method guidelines focus on the PK of traditional drug compounds; small molecules and large biomolecules. The CAR-T (chimeric antigen receptor) cell therapies, recently approved by FDA, involve administration of ‘living drugs’ capable of proliferation after infusion. This behavior is...

To view this content, please register now for access

It's completely free