Application of Mitra® Microsampling for Pharmacokinetic Bioanalysis of Monoclonal Antibodies in Rats
Volumetric absorptive microsampling is being increasingly applied in nonclinical pharmacokinetic studies. Although there are published results for VAM use in small molecule PK studies, there is limited data on the utility of VAM for protein therapeutics.
In this methodology article, H Li et al. describe the use of the Mitra® microsampler for blood sampling, ELISA quantitation and PK analysis of two marketed therapeutic monoclonal antibodies administered to rats. With the increase in the application of volumetric absorptive microsampling (VAM) in nonclinical pharmacokinetic studies, although there are several published results for VAM use in small-molecule PK studies, there is limited data on the utility of VAM for protein therapeutics.
The results generated were compared with both serum and whole blood sampling methods in the same study. The results suggest that Mitra® has the potential to be a convenient and reliable tool for large-molecule analysis in early research animal studies while reducing and refining animal usage.
